High-efficiency transformation of Yarrowia lipolytica using electroporation

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High-throughput transformation method for Yarrowia lipolytica mutant library screening.

As a microorganism of major biotechnological importance, the oleaginous yeast Yarrowia lipolytica is subjected to intensive genetic engineering and functional genomic analysis. Future advancements in this area, however, require a system that will generate a large collection of mutants for high-throughput screening. Here, we report a rapid and efficient method for high-throughput transformation ...

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ژورنال

عنوان ژورنال: FEMS Yeast Research

سال: 2018

ISSN: 1567-1364

DOI: 10.1093/femsyr/foy081